PCCA Knockout cell line(Hep G2)

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PCCA Knockout cell line(Hep G2) Order

PCCA Knockout cell line(Hep G2)
Catalog#
YKO-H514
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PCCA Knockout cell line(Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PCCA Knockout cell line (Hep G2)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
PCCA
Gene ID
5095
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Accession
CVCL_E0UR(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 7 (based on transcript PCCA-202)
Validation: PCR+Sanger Sequencing
PCCA Knockout cell line(Hep G2) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Hep G2		 STR Info (Cell bank)
Cell Line:GS-HepG2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 913913
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XYXY
TPOX 8989
CSF1PO 10111011
D12S391 2125
FGA 2225
D2S1338 1920
D21S11 2931
D18S51 1314
D8S1179 1516
D3S1358 1516
D6S1043 1313
PENTAE 1520
D19S433 15.215.2
PENTAD 913
D1S1656 1112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PCCA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PCCA gene knockout PCCA Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PCCA Knockout cell line(Hep G2)
PCCA Knockout cell line(Hep G2)
Catalog#
YKO-H514
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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